Abstract
ABSTRACTObjectives: To investigate the effect of intake of dairy-products on the sebum lipid components and fatty-acid composition.Patients and Methods: Sebaceous cysts were obtained, by simple surgery, from the scalp, face and neck of 21 men (aged 25-50 y) and divided into two groups. The first group included 11 cysts for subjects consuming dairy products and the second group included 10 cysts for subjects not consuming dairy products. Simple lipids, phospholipids (PLs), triglycerides (TGs), cholesterol (C), cholesterol ester (CE) and fatty acids (FAs) were removed by organic solvents extraction. The sebum lipid components, TGs and C, were determined enzymatically and PLs were determined using a colorimetric method based on the formation of a phosphomolybdate complex. Lipid components were separated by TLC. The separated components were hydrolyzed and their FAs were esterified by super dried-acidified methanol. Fatty-acid methyl esters were identified by capillary gas chromatography.Results: Compared to the non-consumers group, the consumers group exhibited a significant increase in lipid contents, viz, PLs and C. Concerning the FA composition of CE, PLs and TGs, n6-polyunsaturated FAs showed a higher percentage, viz. C18:2 n6 and C9,t11 CLA, while n3-polysaturated FAs showed a significant decrease, viz. C20:5 n3.Conclusion: Consumption of dairy products affects the lipid content and the FA composition of sebum and C9,t11 Conjugated Linoleic Acid could be used as a marker for intake of dairy-product lipid.
