Abstract
Background: A variety of substances, biological and physical interventions have been used to enhance bone fractures healing. There is growing evidences for the importance of nutrition in maintenance of bone health and support of fracture healing.
Objective: The aim of this study was to evaluate the effect of the daily oral dose of concentrated bone broth as dietary supplementation on bone healing in experimental animals (rabbits).
Material and methods: Twelve young male rabbits divided into two groups after induction of open ulnar osteotomy, the experimental group receive daily dose of concentrated bone broth as a dietary supplementation. The control group received ordinary diet. In the end of second weeks three animals from both group were killed, at the end of fifth week, the other animals were sacrificed and the specimens taken for radiological and computerized tomography (CT) scan densimetry and histomorphometric evaluation carried out for the callus at site of osteotomy.
Results: The ulnar bone osteotomies in both groups were united at the end of the fifth week macroscopically and radiologically. The callus density was measured in site of osteotomy by CT scan densimetry, at the end of 2nd week the difference between the experimental and control group was not significant, (P value > 0.05), and at the end of 5th week the difference between the experimental and control group was highly significant, (P value < 0.001). The histomorphometric evaluation of healing in site of osteotomy carried out, and at the end of 2nd week the difference between the experimental and control group was not significant, (P value > 0.05), and at the end of 5th week the difference between the experimental and control group was significant, (P value < 0.05).
Conclusion: The present study demonstrate that a daily oral administration of concentrated bone broth as dietary supplementation in rabbits enhance bone healing by increase callus density in CT, and improve histomorphometric evaluation of healing.
Keywords: Bone broth, fracture, healing, and dietary supplementation.
