Print ISSN: 0027-1446

Online ISSN: 2309-6217

Main Subjects : Pharmacology

Impact of Sacubitril and RAAS inhibitors on p53 expression in rat-induced heart failure. A new approach for ischemic heart disease therapy

Kawa Dizaye; Rojgar Ali; Rafal Al-Rawi

Annals of the College of Medicine, Mosul, 2020, Volume 42, Issue 1, Pages 11-18
DOI: 10.33899/mmed.2020.126595.1021

Background: p53 is a well-known protein that prevents cancer formation, which is recognized as the main protein in the adaptation to many harmful stimuli, like oxidative stress. Among actions of p53, studies have shown that it has an important role in the development of heart failure (HF), and arteriosclerosis. Several clinical studies were done to investigate the role of p53 in the progression of HF and with the intention to improve management of heart failure.
Objective: The purpose of this work was to investigate the mechanisms of myocardial injury that precipitates heart failure that is mediated by both β-adrenergic signaling and p53, then compare the results with administered sacubitril and angiotensin system blockers.
Thirty female albino rats were allocated into five groups; group I: served as a control group; group II: were injected with isoproterenol for HF induction; and groups III, IV, and V (HF treated groups ) whereas rats received sacubitril alone, combination of sacubitril with ramipril and combination of sacubitril with aliskiren respectively, orally on daily basis.
Results revealed that rats of group II (HF induced) were significantly (P = 0.002) showed more myocardial injury and higher nuclear p53 expression compared to rats of the control group. Furthermore, rats of group III, IV, V (HF treated groups) showed significantly (P = 0.037) less myocardial injury and significantly (P = 0.015) less nuclear p53 expression compared to rats of the group II.
It was concluded that rats received either sacubitril alone or with combination of ramipril or aliskiren for HF treatment were alleviated myocardial injury and lower nuclear p53 expression.
It was concluded that anti-p53 approach may provide a novel therapeutic strategy for human ischemic heart diseases and myocardial infarction.

Review study of the role of genetically modified Lactococcus lactis in prevention of type-1 diabetes mellitus

Abeer Mudhafar Al-Humaidhi; Ahmed Abdulkareem Shegaya; Ali Qusay Sagban; Hussain Ali Jaleel; Karrar Mahtar Ateha; Maitham Jaber Abdulhussain

Annals of the College of Medicine, Mosul, 2020, Volume 42, Issue 1, Pages 82-89
DOI: 10.33899/mmed.2020.127468.1040

Globally, type 1 diabetes mellitus is a common chronic autoimmune disease impacts a great number of individuals. It results from devastation of insulin-secreting beta cells of the pancreas described by incompetence to secrete insulin resulting in hyperglycemia that need a ceaseless treatment with insulin. Further, complications of type 1 diabetes can influence numerous organs and may prompt premature death. Therefore, the requirement to find new strategies for managing type 1 diabetes other than exogenous insulin administration got obligatory. Curiously, genetically modified Lactococcus lactis can incite immunological tolerance; downregulate mechanisms linked to adaptive immunity; and reduce disease-related inflammation, while leaving the remainder of the immune system flawless. This review aimed to overview genetically modified Lactococcus lactis, it's expressing proteins, pathogenesis of type 1diabetes, and therapeutic effects of genetically modified Lactococcus lactis in animal model of type 1daibetes, further, to display the possible mechanisms through which the genetically modified Lactococcus lactis can act. It has been presumed that targeting antigen-specific pathway utilizing genetically modified Lactococcus lactis has potential therapeutic impacts in experimental animals with type 1 diabetes mellitus. This could open up new and safe therapeutic prospects in autoimmune disease domain such as type 1diabetes mellitus.