Print ISSN: 0027-1446

Online ISSN: 2309-6217

Main Subjects : Microbiology

susceptibility of yeast species to different concentration to fluconazole in leukemic and non- leukemic cancer patients

Zahraa Qasim

Annals of the College of Medicine, Mosul, 2020, Volume 42, Issue 1, Pages 42-49
DOI: 10.33899/mmed.2020.127200.1027

Aim : Isolation and identification of yeast species in leukemic and non leukemic cancer patients and conduct the in vitro fluconazole susceptibility in different concentrations.
Patients and methods: This study included 100 oral swabs samples from cancer patients 50 of them were leukemic cancer patients group I, while group II patients were 50 non-leukemic cancer patients. Group I patients attending the hematology department in Ibn-Sina teaching hospital, and group II patients attending oncology and nuclear medicine teaching hospital in Mosul city. The clinical specimens were collected during period (June - December 2013).The patients included in this study were females only. Isolation and identification of yeast species depend on the classical methods. All the isolated yeast species were tested for their susceptibility to fluconazole concentrations (flu.10mcg, flu. 25mg, flu 50mcg) using standard disc agar diffusion method.
Results: C. albicans 28(52.8%), C. topicalis 1(1.9%), Cryptococcus laurentii 1(1.9%) all of them were higher in group I than group II, in addition C.parasilosis 1(1.9%) was higher in group II than group I, while C. glabrata was the same in group I 1(1.9%) and group II 1(1.9%). Fluconazole sensitivity test ranged from 40(38.1%), 34(32.4%), 31(29.5) to fluconazole discs potency 50mcg, 25mcg, 10mcg in both groups of cancer patients.
Conclusion: C. albicans, C. topicalis, Cryptococcus laurentii all of them were higher in group I than group II, in addition C.parasilosis was higher in group II than group I, while C. glabrata was the same in group 1 and group II. The sensitivity test to fluconazole were dose dependent with no significant difference between the three concentration to fluconazole

The Role of Vitamin C on the Structural Changes of Male Albino Rats Kidney Induced by Tramadol

Rana M. Ahmad; Abduljabbar Y. AL-Hubaity; Nadwa S. Alazow

Annals of the College of Medicine, Mosul, 2019, Volume 41, Issue 1, Pages 57-62
DOI: 10.33899/mmed.2019.161285

Aim of study: The aim of this work is to detect the toxic effects of tramadol on the microscopic structure of the kidneys of adult male albino rats, and the possible protective role of vitamin C against these toxic effects.
Method: Thirty adult albino rats are used in this study. The animals were divided into three equal groups: Group A (control group): Animals were injected daily with normal saline for 4 weeks. Group B (Tramadol treated group) (TGI): Animals were injected daily with tramadol in a dose of 50mg/kg b.wt for 4 weeks. Group C (group treated with tramadol+vitamin C) (TGII): Animals were injected with vitamin C in a dose 100mg/Kg/b.wt half hour prior to tramadol injection in the same dose as in group B for 4 weeks. The injection was given intraperitoneally for all animals. At the end of the experiments, the rats were anaesthetized by ether, then killed. After that kidneys removed, fixed in formaldehyde and processed for microscopic examination.
Result: Tramadol causes shrinkage of renal glomeruli with widening of bowman's space. Renal tubules showed hydropic degeneration and vacuolization of their epithelial cells. Infiltration of mononuclear cells along with Hyaline cast is seen within the degenerated tubules. Giving vitamin C prior to tramadol injection results  in improvement in the microscopical structure of kidney mainly in glomeruli and tubules.
Conclusion: Using tramadol caused damage to renal glomeruli and tubules in renal cortex. The addition of vitamin C partially improved the histological structure of kidney.